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Title: Hair transplantation in mice: challenges and solutions.
Epworth Authors: Rufaut, Nicholas
Jones, Leslie
Sinclair, Rodney
Other Authors: Asgari, Azar
Morrison, Wayne
Dilley, Rodney
Knudsen, Russle
Keywords: Hair Follicle
Hair Follicle Transplantation
Wound Healing
Mouse Model
Follicular Unit Transplant
Vibrissa Follicle Transplant
Hair Transplantation Methodology
Flip-side Hair Follicle Microdissection
Head & Neck Clinical Institute, Epworth HealthCare
Chair of Dermatology, Epworth HealthCare
Issue Date: Apr-2016
Publisher: Wiley
Citation: Wound Repair Regen. 2016
Abstract: Hair follicle cells contribute to wound healing, skin circulation, and skin diseases including skin cancer, and hair transplantation is a useful technique to study the participation of hair follicle cells in skin homeostasis and wound healing. Although hair follicle transplantation is a well-established human hair-restoration procedure, follicular transplantation techniques in animals have a number of shortcomings and have not been well described or optimized. To facilitate the study of follicular stem and progenitor cells and their interaction with surrounding skin, we have established a new murine transplantation model, similar to follicular unit transplantation in humans. Vibrissae from GFP transgenic mice were harvested, flip-side microdissected, and implanted individually into needle hole incisions in the back skin of immune-deficient nude mice. Grafts were evaluated histologically and the growth of transplanted vibrissae was observed. Transplanted follicles cycled spontaneously and newly formed hair shafts emerged from the skin after 2 weeks. Ninety percent of grafted vibrissae produced a hair shaft at 6 weeks. After pluck-induced follicle cycling, growth rates were equivalent to ungrafted vibrissae. Transplanted vibrissae with GFP-positive cells were easily identified in histological sections. We established a follicular vibrissa transplantation method that recapitulates human follicular unit transplantation. This method has several advantages over current protocols for animal hair transplantation. The method requires no suturing and minimizes the damage to donor follicles and recipient skin. Vibrissae are easier to microdissect and transplant than pelage follicles and, once transplanted, are readily distinguished from host pelage hair. This facilitates measurement of hair growth. Flip-side hair follicle microdissection precisely separates donor follicular tissue from interfollicular tissue and donor cells remain confined to hair follicles. This makes it possible to differentiate migration of hair follicle cells from interfollicular epidermis in lineage tracing wound experiments using genetically labeled donor follicles.
Description: Online version of record published before inclusion in an issue.
DOI: 10.1111/wrr.12435
PubMed URL:
ISSN: 1524-475X
Journal Title: Wound Repair and Regeneration
Type: Journal Article
Affiliated Organisations: Department of Medicine (SVHM), The University of Melbourne, Melbourne
O'Brien Institute, St. Vincent's Institute, Fitzroy, Melbourne
Department of Surgery (SVHM), the University of Melbourne, Melbourne
Ear Science Institute Australia, and Ear Sciences Centre, University of Western Australia, Western Australia
The Knudsen Clinic, Sydney, Australia
Type of Clinical Study or Trial: Prospective Study
Appears in Collections:Dermatology
Head & Neck

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