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Title: A simple hair transplantation model to investigate follicular cells and their interaction with surrounding skin.
Epworth Authors: Rufaut, Nicholas
Sinclair, Rodney
Other Authors: Asgari, Azar
Jones, Leslie
Keywords: Microdissection
Dendritic Cells, Follicular
Follicular Dendritic Cells
Wound Healing
Epithelial Cells
GFP Positive Cells
Skin Circulation
Skin Diseases
Hair Transplantation
Hair Follicle Cells
Skin Homeostasis
Hair Restoration Procedure
Follicular Transplantation Techniques
Hair Growth
Department of Dermatology, Epworth Hospital, Richmond, Victoria, Australia.
Epworth Research Institute, Richmond, Victoria, Australia.
Issue Date: Jul-2016
Citation: Epworth Research Institute Research Week 2016; Poster 1: pp 20
Conference Name: Epworth Research Institute Research Week 2016
Conference Location: Richmond, Victoria, Australia.
Abstract: INTRODUCTION: Hair follicle cells contribute to wound healing, skin circulation, and skin diseases and hair transplantation is a useful technique to study the participation of hair follicle cells in skin homeostasis and wound healing. BACKGROUND: Although hair follicle transplantation is a well-established human hair restoration procedure, follicular transplantation techniques in animals have not been well described or optimized. METHOD: Vibrissae from GFP transgenic mice were harvested, micro-dissected and implanted individually into needle hole incisions in the back skin of immune-deficient nude mice. Grafts were evaluated histologically and the growth of transplanted vibrissae was observed. Migration of GFP positive cells from transplanted follicles into the inter-follicular epidermis was investigated by histological examination in animals without a wound (5 animals) and animals with an incisional wound (2 animals). RESULTS: Transplanted follicles cycled spontaneously. Ninety percent of grafted vibrissae produced a hair shaft at 6 weeks. after pluck-induced follicle cycling, growth rates were equivalent of ungrafted vibrissae. Histology examination after 6 weeks showed that transplanted follicle were innervated and connected to the host circulation system. All observed GFP-positive cells stayed in the hair follicles and had not moved into the surrounding tissue. In animals receiving an incisional would however, histology examination on day 4 showed that GFP positive cells had migrated into the inter-follicular epidermis. CONCLUSION: Established follicular vibrissae transplantation method has several advantages over current protocols for animals hair transplantation. Vibrissae are easier to micro-dissect and transplant than pelage follicles and once transplanted, are readily distinguished from host pelage hair. This facilitates measurement of hair growth. Flip-side hair follicle micro-dissection precisely separates the donor follicular tissue from inter-follicular tissue and donor cells remain confined to hair follicles. This makes it possible to investigate hair follicle epithelial cell migration during wound re-epithelialization and differentiate migrating hair follicle cells from inter-follicular epidermis in linage tracing wound experiments using genetically labelled donor follicles.
Type: Conference Poster
Affiliated Organisations: O'Brien Institute, St Vincent's Institute, Fitzroy, Victoria, Australia.
Department of Medicine, The University of Melbourne, Parkville, Victoria, Australia.
Type of Clinical Study or Trial: Descriptive Study
Appears in Collections:Dermatology
Head & Neck
Research Month

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