Please use this identifier to cite or link to this item:
|Title:||Mapping the testicular interstitial fluid proteome from normal rats.|
|Epworth Authors:||Stephens, Andrew|
|Other Authors:||Stanton, Peter|
Testicular Interstitial Fluid
Epworth Research Institute, Epworth Healthcare, Richmond, Victoria, Australia.
|Citation:||Proteomics. 2016 Jun 21.|
|Abstract:||Communication between the testicular somatic- (Sertoli, Leydig, peritubular myoid, macrophage) and germ- cell types is essential for sperm production (spermatogenesis), but the communicating factors are poorly understood. We reasoned that identification of proteins in the testicular interstitial fluid (TIF) that bathes these cells could provide a new means to explore spermatogenic function. The aim of this study was to map the proteome of TIF from normal adult rats. Low-abundance proteins in TIF were enriched using Proteominer beads, and identified by MALDI tandem mass spectrometry, recognising 276 proteins. Comparison with proteomic and genomic databases showed these proteins originated from germ cells, somatic cells (Sertoli, peritubular myoid, Leydig) and blood plasma. In silico analysis revealed homologues of >80% TIF proteins in the human plasma proteome, suggesting ready exchange between these fluids. Only 36% of TIF proteins were common with seminiferous tubule fluid that transports mature spermatids to the epididymis, indicating these two fluids are quite different. This TIF proteome provides an important new resource for the study of intercellular communication in the testis.|
|Affiliated Organisations:||Hudson Institute of Medical Research, Clayton, Victoria, Australia.|
Department of Molecular and Translational Science, Monash University, Clayton, Victoria 3800, Australia.
Bruker Pty Ltd, Preston, Victoria, Australia.
Department of Urology, Paediatric Urology and Andrology, Justus Liebig University, Giessen, Germany.
Department of Urology, Ishikawa Hospital, Himeji, Japan.
MRC Centre for Reproductive Health, University of Edinburgh, United Kingdom.
|Appears in Collections:||UroRenal, Vascular|
Files in This Item:
There are no files associated with this item.
Items in EKB are protected by copyright, with all rights reserved, unless otherwise indicated.