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Title: Culture of dermal papilla cells from ovine wool follicles: an in vitro model for papilla size determination.
Epworth Authors: Sinclair, Rodney
Other Authors: Rufaut, Nicholas
Nixon, Allan
Keywords: Hair Follicle
Dermal Papilla
Hair Loss
In Vitro Model
Follicle Miniaturization
Papilla Size
Head & Neck Clinical Institute, Epworth HealthCare
Chair of Dermatology, Epworth HealthCare
Issue Date: Jul-2016
Publisher: Springer
Citation: Methods Mol Biol. 2016;1453:109-21
Abstract: Common human balding or hair loss is driven by follicle miniaturization. Miniaturization is thought to be caused by a reduction in dermal papilla size. The molecular mechanisms that regulate papilla size are poorly understood, and their elucidation would benefit from a tractable experimental model. We have found that dermal papilla cells from sheep spontaneously aggregate in culture to form papilla-like structures. Here, we describe methods for microdissecting dermal papillae from wool follicles, for initiating and maintaining cultures of ovine papilla cells, and for using these cells in an in vitro assay to measure the effect of bioactive molecules on aggregate size.
Description: Part of monograph in series 'Multipotent stem cells of the hair follicle : methods and protocols' /​ edited by Robert M. Hoffman. Print ISBN 978-1-4939-3784-4, online ISBN 978-1-4939-3786-8.
DOI: 10.1007/978-1-4939-3786-8_12.
PubMed URL:
ISSN: 1064-3745
Journal Title: Methods in Molecular Biology
Type: Journal Article
Affiliated Organisations: Department of Medicine, University of Melbourne, Melbourne, Australia
Histopathology Department, Waikato District Health Board, Hamilton, New Zealand
Appears in Collections:Head & Neck

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